ATP citrate lyase, aslo known as ACLY,is anenzymethat in animals represents an important step infatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis ofcytosolicacetyl-CoAin many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, includinglipogenesis andcholesterogenesis.It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Mouse
Immunogen Region
A human recombinant protein (amino acids M1-I180) was used as the immunogen for the ATP Citrate Lyase antibody.
Isotype
IgG2b
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Mouse, Rat, Human
Recombinant
No
Antigen
ATP Citrate Lyase
Uniprot
P53396
Clone No
5I2
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Antigen affinity purified
Purification
Affinity purified
Storage
After reconstitution, the ATP Citrate Lyase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-P, FACS, WB, IF
Dilution
Western blot: 0.1-0.5ug/ml,Immunohistochemistry(FFPE): 0.5-1ug/ml,Flow Cytometry: 1-3ug/1x10^6 cells,Immunofluorescence: 2-4ug/ml