Argininosuccinate synthetase is an enzyme that in humans is encoded by the ASS1 gene. It is mapped to 9q34.11. The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Mouse
Immunogen Region
A human recombinant partial protein (amino acids S3-S365) was used as the immunogen for the Argininosuccinate Synthetase 1 antibody.
Isotype
IgG2a
Predicted Reactivity
Human, Mouse, Rat, Monkey
Reactivity
Mouse, Rat, Monkey, Human
Recombinant
No
Subcellular Location
Cytoplasmic
Antigen
ASS1
Uniprot
P00966
Clone No
5I5
Format
Antigen affinity purified
Purification
Affinity purified
Storage
After reconstitution, the Argininosuccinate Synthetase 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-P, IF, WB, FACS
Dilution
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells