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Home / Primary Antibodies / Monoclonal Antibodies

Anti-p53 Antibody

SKU : BHA17127933

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NSJ Bioreagents

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Mfr.No.	V9090IHC, V9090IHC-7ML
Clonality	Monoclonal (mouse origin)
Description	Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Formulation	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only
Host	Mouse
Immunogen Region	Recombinant human wild type p53 protein was used as the immunogen for this anti-p53 antibody. Its epitope maps within the N-terminus (approx. amino acids 37-45).
Isotype	Mouse IgG2b kappa
Species Reactivity	Human
Note	Optimal dilution of the antibody should be determined by the researcher.

Uniprot	P04637

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the anti-p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Product Manual	<a href="https://www.nsjbio.com/tds-pdf/anti-p53-antibody-spm590-v9090" target="_blank">Datasheet</a>

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SKU	Supplier No.	Size	Your price	Quantity
BHA17127933	V9090IHC, V9090IHC-7ML	7ml	$539		Add to Cart

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