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Home / Primary Antibodies / Monoclonal Antibodies

Anti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3)

SKU : BHA21002587

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Mfr.No.	M00409-2, M00409-2-10ug, M00409-2-Biotin, M00409-2-Cy3, M00409-2-Dylight488, M00409-2-Dylight550, M00409-2-Dylight594, M00409-2-FITC, M00409-2-HRP, M00409-2-APC, M00409-2-PE, M00409-2-iFluor647, M00409-2-carrier-free
Alternative Names	tripartite motif containing 28
Background	Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1beta (TIF1beta) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3) catalog # M00409-2. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at '
Host	Mouse
Immunogen Region	E.coli-derived human KAP1 recombinant protein (Position: A699-P835). Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1.
Isotype	Mouse IgG2a
Molecular Weight	100 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	E3 SUMO protein ligase TRIM28 antibody; E3 SUMO-protein ligase TRIM28 antibody; FLJ29029 antibody; KAP 1 antibody; KAP-1 antibody; KRAB associated protein 1 antibody; KRAB interacting protein 1 antibody; KRAB-associated protein 1 antibody; KRAB-interacting protein 1 antibody; KRIP 1 antibody; KRIP-1 antibody; KRIP1 antibody; Nuclear corepressor KAP 1 antibody; Nuclear corepressor KAP-1 antibody; RING finger protein 96 antibody; RNF96 antibody; TF1B antibody; TIF1 beta antibody; TIF1-beta antibody; TIF1B antibody; TIF1B_HUMAN antibody; Transcription intermediary factor 1 beta antibody; Transcription intermediary factor 1-beta antibody; Trim28 antibody; Tripartite motif containing 28 antibody; tripartite motif containing protein 28 antibody; Tripartite motif-containing protein 28 antibody
Tissue Specificity	Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.

Uniprot	Q13263
Gene Id	10155
Gene Name	TRIM28
Clone No	Clone: 9E3
Subcellular Location	Nucleus.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, IHC-F, ICC, IF, Flow Cytometry
Description	Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Frozen Section), 0.5-1ug/ml, Human, Rat<br> Immunocytochemistry/Immunofluorescence, 2ug/ml, Human<br> Immunofluorescence, 2ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br>

Reference	1. Capili AD, Schultz DC, RauscherIII FJ, Borden KL (Jan 2001). "Solution structure of the PHD domain from the KAP-1 corepressor: structural determinants for PHD, RING and LIM zinc-binding domains". The EMBO Joual 20 (1-2): 16577.<br /><br />2. Iyengar, Sushma; Faham, Peggy (2011-07-29). "KAP1 Protein: An Enigmatic Master Regulator of the Genome". The Joual of Biological Chemistry 286.<br /><br />3. Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A (May 2001). "The tripartite motif family identifies cell compartments". The EMBO Joual 20 (9): 214051.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002587-1	M00409-2	-	100 ug	$370		Add to Cart
BHA21002587-2	M00409-2-10ug	Sample	10 ug	$99		Add to Cart
BHA21002587-3	M00409-2-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002587-4	M00409-2-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002587-5	M00409-2-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002587-6	M00409-2-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002587-7	M00409-2-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002587-8	M00409-2-FITC	FITC	100 ug	$570		Add to Cart
BHA21002587-9	M00409-2-HRP	HRP	100 ug	$570		Add to Cart
BHA21002587-10	M00409-2-APC	APC	100 ug	$670		Add to Cart
BHA21002587-11	M00409-2-PE	PE	100 ug	$670		Add to Cart
BHA21002587-12	M00409-2-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002587-13	M00409-2-carrier-free	carrier-free	100 ug	$370		Add to Cart

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