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Home / Primary Antibodies / Monoclonal Antibodies

Anti-IgG Antibody

SKU : BHA17123481

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NSJ Bioreagents

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Mfr.No.	V2140SAF, V2140SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	Immunoglobulin gamma (IgG) is the most common class of antibody in blood and extracellular fluid. Approximately 75% of serum antibodies in humans are IgG. There are four immunoglobulin gamma subclasses: one, two, three and four. IgG1 is the most common, with 68% of all gamma class antibodies being G1, and G4 is the least common at 4%. Gamma class antibodies are found primarily in the secondary immune response, class switching from IgM and IgD. They are the only class of antibody that can cross the placenta, and along with IgA secreted in breast milk, provide the neonate with humoral immunity before immune system development occurs.This antibody recognizes a protein of 75kDa identified as the gamma heavy chain of human immunoglobulins. It does not cross-react with alpha, mu, epsilon, or delta heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The IgG antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Purified human IgG heavy chain was used as the immunogen for this anti-IgG antibody.
Isotype	Mouse IgG2a, kappa
Species Reactivity	Human
Note	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-IgG antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the anti-IgG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	FACS, IF, IHC-P
Description	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17123481	V2140SAF, V2140SAF-100UG	100ug	$539		Add to Cart

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