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Home / Primary Antibodies / Monoclonal Antibodies

Anti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4)

SKU : BHA21002256

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Mfr.No.	M00223-1, M00223-1-10ug, M00223-1-Biotin, M00223-1-Cy3, M00223-1-Dylight488, M00223-1-Dylight550, M00223-1-Dylight594, M00223-1-FITC, M00223-1-HRP, M00223-1-APC, M00223-1-PE, M00223-1-iFluor647, M00223-1-carrier-free
Alternative Names	SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Background	Transcription activator BRG1 also known as ATP-dependent helicase SMARCA4 is a protein that in humans is encoded by the SMARCA4 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4) catalog # M00223-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structur
Host	Mouse
Immunogen Region	E. coli-derived human BRG1 recombinant protein (Position: Q555-E763).
Isotype	Mouse IgG1
Molecular Weight	181 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Cyclin-dependent kinase 1; CDK1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDK1; CDC2P; CDC28A; CDKN1; P34CDC2
Tissue Specificity	Colocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level).
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	P51532
Gene Id	6597
Gene Name	SMARCA4
Clone No	Clone: 3F4
Subcellular Location	Nucleus.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IHC, WB
Description	Weste blot, 0.1-0.5ug/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells<br>

Reference	1. "Entrez Gene: SMARCA4 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4".<br /><br />2. DiRenzo J, Shang Y, Phelan M, Sif S, Myers M, Kingston R, Brown M (October 2000). "BRG-1 is recruited to estrogen-responsive promoters and cooperates with factors involved in histone acetylation". Molecular and Cellular Biology. 20 (20): 7541-9.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002256-1	M00223-1	-	100 ug	$370		Add to Cart
BHA21002256-2	M00223-1-10ug	Sample	10 ug	$99		Add to Cart
BHA21002256-3	M00223-1-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002256-4	M00223-1-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002256-5	M00223-1-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002256-6	M00223-1-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002256-7	M00223-1-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002256-8	M00223-1-FITC	FITC	100 ug	$570		Add to Cart
BHA21002256-9	M00223-1-HRP	HRP	100 ug	$570		Add to Cart
BHA21002256-10	M00223-1-APC	APC	100 ug	$670		Add to Cart
BHA21002256-11	M00223-1-PE	PE	100 ug	$670		Add to Cart
BHA21002256-12	M00223-1-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002256-13	M00223-1-carrier-free	carrier-free	100 ug	$370		Add to Cart

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