Mouse IL1b(Interleukin 1 Beta) Microsample ELISA Kit

Mfr.No.

ELK1271MS

Sensitivity

6.4 pg/mL

Detection Range

15.63-1000 pg/mL

Reactivity

Mouse

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

IL-1B;IL1-B; IL1-Beta; IL1F2; IL-1beta; Interleukin-1 Family Member 2; Catabolin

Recovery

Matrices listed below were spiked with certain level of recombinant IL1b and the recovery rates were calculated by comparing the measured value to the expected amount of IL1b in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	81-93%	87%
Heparin plasma(n=5)	90-105%	97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	83-98%	87-103%	89-97%	85-94%
EDTA plasma(n=5)	95-103%	79-97%	93-102%	87-103%
Heparin plasma(n=5)	85-93%	79-96%	93-106%	85-99%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.136	2.048
500.00	1.629	1.541
250.00	1.182	1.094
125.00	0.933	0.845
62.50	0.589	0.501
31.25	0.357	0.269
15.63	0.234	0.146
0.00	0.088	0.000

Sample Type

Cell lysates, Serum, Cell culture supernatants, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

1000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P10749

Applications	ELISA
Research Area	Cytokine;Tumor immunity;Infection immunity;

Product Components

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208364	ELK1271MS	48T	$320		Add to Cart
BHE15208364	ELK1271MS	96T	$458		Add to Cart

Mouse IL1b(Interleukin 1 Beta) Microsample ELISA Kit

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