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ELK Biotechnology
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ELK Biotechnology
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| Mfr.No. | ELK3339MS | |||||||||||||||||||||||||||
| Sensitivity | 14.3 pg/mL | |||||||||||||||||||||||||||
| Detection Range | 31.25-2000 pg/mL | |||||||||||||||||||||||||||
| Reactivity | Mouse | |||||||||||||||||||||||||||
| Assay Duration | 3.5h | |||||||||||||||||||||||||||
| Assay Type | Sandwich | |||||||||||||||||||||||||||
| Alternative Names | DEF1; DEFA2; HNP-1; HP-1; MRS; DEF3; HNP-3; HNP3; HP-3; Defensin,Alpha 1; Myeloid-Related Sequence | |||||||||||||||||||||||||||
| Kit Components |
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| Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DEFa1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| Standard Curve |
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| Sample Type | Serum, Plasma, Tissue homogenates, Other biological fluids | |||||||||||||||||||||||||||
| Limit Of Detection | 2000 pg/mL | |||||||||||||||||||||||||||
| Precision |
Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. |
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| Usage Notes | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DEFa1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DEFa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DEFa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DEFa1 in the samples is then determined by comparing the OD of the samples to the standard curve. | |||||||||||||||||||||||||||
| Protocol | Manual |
| SKU | Supplier No. | Size | Your price | Quantity | |
|---|---|---|---|---|---|
| BHE15211748 | ELK3339MS | 48 T | $320 |
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| BHE15211748 | ELK3339MS | 96 T | $458 |
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| BHE15211748 | ELK3339MS | 96 T*5 | $1947 |
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