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Home / VECTOR &VIRUS / Lentivirus

MyoD Reporter Lentivirus

SKU : BHV19400045

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Mfr.No.	LTV-0049, LTV-0049-1S, LTV-0049-2S, LTV-0049-3S, LTV-0049-4S, LTV-0049-5S, LTV-0049-6S, LTV-0049-4SIC
Description	Fluorescent MyoD Reporter Lentivirus: High-quality, fluorescent lentiviral reporter system for the readout of myoblast determination protein 1 (MyoD) in mammalian cells. Tandem repeats of the response element contain muscle creatine kinase (MCK)-L and MCK-R sites, which are the DNA-binding motifs recognized by myogenic factors such as MyoD, Myf5, Myogenin, and MRF4. MyoD and Myf5 are active in proliferating myoblasts, whereas Myogenin and MRF4 occur after myoblast exit from the cell cycle. This product could be used for monitoring the transcriptional activities of myogenic factors in muscle cells. The lentiviral particles are purified by PEG precipitation and sucrose gradient centrifugation, and are ideal for studying the activity of myogenic factors such as MyoD in difficult-to-transfect cells including primary and/or thawed cells.
Promoter	EF1a
Serotype	VSV-G
Reporter	Firefly Luc, RFP, Renilla Luc, GFP
Response Element	Tandem repeats containing muscle creatine kinase (MCK)-L and MCK-R sites
Selection	Blasticidin, Puromycin
Signaling pathway	Myogenic factors, MyoD
Species	Human/mouse
Applications Range	Monitor activity of myogenic factors (such as MyoD, Myf5, Myogenin, MRF4, etc.) in mammalian cells, screen for activators or inhibitors of myogenic factors. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. MyoD and Myf5 are active in proliferating myoblasts, whereas Myogenin and MRF4 occur after myoblast exit from the cell cycle.
Virus Type	TF Reporter Lentivirus

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Combination SKU	Supplier No.	Amount (TU)	Selection	Reporter	Promoter	Your price	Quantity
BHV19400045-1	LTV-0049-1S	5x10^6	Puromycin	GFP	EF1a	$595		Add to Cart
BHV19400045-2	LTV-0049-2S	5x10^6	Blasticidin	RFP	EF1a	$595		Add to Cart
BHV19400045-3	LTV-0049-3S	2x10^6	Puromycin	Firefly Luc	EF1a	$595		Add to Cart
BHV19400045-4	LTV-0049-4S	2x10^6	Blasticidin	Firefly Luc	EF1a	$595		Add to Cart
BHV19400045-5	LTV-0049-5S	5x10^6	Blasticidin	GFP	EF1a	$595		Add to Cart
BHV19400045-6	LTV-0049-6S	5x10^6	Puromycin	RFP	EF1a	$595		Add to Cart
BHV19400045-7	LTV-0049-4SIC	2x10^6	Blasticidin	Renilla Luc	EF1a	$595		Add to Cart

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