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Home / VECTOR &VIRUS / Lentivirus

KRAS-G12D Human ORF cDNA Lentivirus

SKU : BHV19400101

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Mfr.No.	LCV-0009-1S, LCV-0009-2S, LCV-0009-3S, LCV-0009-4S
Description	Human KRAS G12D ORF cDNA lentivirus. Lentiviral particles, packaged with expression vector for G12D mutant of human KRAS proto-oncogene, GTPase (KRAS), transcript variant a. An EF1? promoter drives expression of KRAS G12D fused to a C-terminal V5 tag, and a GFP reporter which is separated from the ORF cDNA by a self-cleaving peptide.<br />These lentiviral particles allow for easy transduction and high stable expression of the indicated ORF cDNA in mammalian cells. The lentiviral vectors have been optimized to provide high gene expression and reliable genome integration, allowing for easy establishment of long-term, stable cell lines with consistent read out of fluorescent (GFP, RFP) or luminescent (firefly luc) reporters. Transcription of the ORF cDNA fused with C-terminal epitope tag is controlled by either EF1a or CMV promoter, while expression of the reporter or selection marker (puromycin or blasticidin) is separated by a self-cleaving peptide to allow separate translation via the same mRNA transcript. In some constructs, the CMV or EF1a promoter drives expression of cDNA-Tag, while the GFP/RFP reporter and drug selection marker are driven by PGK promoter and separated by a self-cleaving peptide. The particles are capable of transducing a variety of difficult-to-transfect cells, including primary and/or thawed cells, making them an extremely versatile product for studying genes in mammalian systems. The accuracy of ORF cDNA cloning has been confirmed by sequencing and the successful protein expression has been validated by transient transfection.
Promoter	EF1a
Serotype	VSV-G
Fusion Protein Or Tag	Puromycin, Blasticidin
Reporter	V5, Flag
Selection	GFP, RFP
Species	Human
Symbol	KRAS
Virus Type	cDNA Lentivirus

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Combination SKU	Supplier No.	Size	Epitope tag	Selection	Reporter	Promoter	Your price	Quantity
BHV19400101-1	LCV-0009-1S	2x10^6	N/A	GFP	V5	EF1a	$595		Add to Cart
BHV19400101-2	LCV-0009-2S	2x10^6	N/A	RFP	V5	EF1a	$595		Add to Cart
BHV19400101-3	LCV-0009-3S	2x10^6	Puromycin	N/A	Flag	EF1a	$595		Add to Cart
BHV19400101-4	LCV-0009-4S	2x10^6	Blasticidin	N/A	Flag	EF1a	$595		Add to Cart

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