GLI Reporter Lentivirus (Hedgehog Pathway)

Mfr.No.	LTV-0012, LTV-0012-1S, LTV-0012-2S, LTV-0012-3S, LTV-0012-4S, LTV-0012-5S, LTV-0012-6S, LTV-0012-4SIC
Description	Fluorescent GLI Reporter Lentivirus (Hedgehog Pathway): High-quality, fluorescent lentiviral reporter system for Hedgehog pathway activity in mammalian cells. Tandem repeats of the consensus GLI response element drive the fluorescent reporter to readout transcriptional activity of GLI family TFs in response to Hedgehog pathway ligands, such as sonic hedgehog (Shh), indian hedgehog (Ihh), and desert hedgehog (Dhh). The GLI family is composed of the activator GLI1, and the dual activity transcription factors GLI2 and GLI3. The lentiviral particles are purified by PEG precipitation and sucrose gradient centrifugation, and are ideal for studying activation of GLI/Hedgehog pathway in difficult-to-transfect cells including primary and/or thawed cells.
Promoter	EF1a
Serotype	VSV-G
Reporter	Firefly Luc, RFP, Renilla Luc, GFP
Response Element	GLI response element
Selection	Blasticidin, Puromycin
Signaling pathway	GLI family TFs and Hedgehog pathways (e.g. Shh, Ihh, Dhh)
Species	Human/mouse
Applications Range	Monitor GLI/Hedgehog pathway activity in mammalian cells. The reporter could be useful for studying inhibitors of oncogenic GLI/Hedgehog pathway in brain tumors. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc.
Virus Type	TF Reporter Lentivirus

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Combination SKU	Supplier No.	Amount (TU)	Selection	Reporter	Promoter	Your price
BHV19400011-1	LTV-0012-1S	5x10^6	Puromycin	GFP	EF1a	$595	Add to Cart
BHV19400011-2	LTV-0012-2S	5x10^6	Blasticidin	RFP	EF1a	$595	Add to Cart
BHV19400011-3	LTV-0012-3S	2x10^6	Puromycin	Firefly Luc	EF1a	$595	Add to Cart
BHV19400011-4	LTV-0012-4S	2x10^6	Blasticidin	Firefly Luc	EF1a	$595	Add to Cart
BHV19400011-5	LTV-0012-5S	5x10^6	Blasticidin	GFP	EF1a	$595	Add to Cart
BHV19400011-6	LTV-0012-6S	5x10^6	Puromycin	RFP	EF1a	$595	Add to Cart
BHV19400011-7	LTV-0012-4SIC	2x10^6	Blasticidin	Renilla Luc	EF1a	$595	Add to Cart