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Home / Primary Antibodies / Monoclonal Antibodies

LAMP2 Antibody, Clone GL2A7 (SMC-141)

BHA11900600
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Antigen LAMP2
Swissprot P17047
Accession Number NP_001017959.1
Gene Id 16784
Target LAMP2
Clone No GL2A7
Research Area Cell Signaling, Tags and Cell Markers, Organelle Markers, Chaperone Proteins, Neuroscience, Protein Trafficking
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3).
LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4).
Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C4 and prostaglandin D2, which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~100-110kDa.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IP, WB, ICC/IF
Description WB ; ICC ; IF ; IP
Dilution WB (1:1000), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Granger B.L., et al. (1990) J. Biol. Chem. 265: 12036-12043.

2. Furuta K., et al. (1999) EMBO J. 17(5):1304-14.

3. Rohrer J., et al. (1996) J Cell Biol. 132(4): 565-76.

4. Lichter-Konecki U., et al (1999) Differentiation 65(1): 43-58.

5. Grutzkau A., et al. (2004) Cytometry A. 61(1): 62-68.

6. Kannan K., et al. (1996) Cell Immunol. 171: 10-19.

7. Tanaka Y., et al. (2000) Nature 406: 902-906.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900600-1 SMC-141D Unconjugated 100 ug $313
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BHA11900600-2 SMC-141D-A390 ATTO 390 100 ug $368
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BHA11900600-3 SMC-141D-A488 ATTO 488 100 ug $367
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BHA11900600-4 SMC-141D-A594 ATTO 594 100 ug $367
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BHA11900600-5 SMC-141D-APC APC 100 ug $366
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BHA11900600-6 SMC-141D-BI Biotin 100 ug $362
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BHA11900600-7 SMC-141D-FITC FITC 100 ug $358
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BHA11900600-8 SMC-141D-HRP HRP 100 ug $354
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BHA11900600-9 SMC-141D-PCP PerCP 100 ug $366
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BHA11900600-10 SMC-141D-RPE RPE 100 ug $363
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BHA11900600-11 SMC-141S Unconjugated 12 ug $45
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