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Home / Primary Antibodies / IHC Antibodies

VIM Antibody

SKU : BHA10559789

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA233606
Alternative Names	VIME; vimentin;
Categories	Primary Antibodies
Clonality	polyclonal
Description	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.WB Isaacs, J. Biol. Chem., Oct 1989; 264: 17953 - 17960. Patric Turowski, Mol. Biol. Cell, Jun 1999; 10: 1997. Melanie L. Styers, J. Cell Sci., Sep 2006; 119: 3643 - 3654. Qing-Fen Li, J. Biol. Chem., Nov 2006; 281: 34716 - 34724.
Host	Rabbit
Immunogen	Synthesized peptide derived from Internal of Human vimentin.
Involvement In Disease	Cataract 30, multiple types (CTRCT30)
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. WB Isaacs, J. Biol. Chem., Oct 1989; 264: 17953 - 17960. Patric Turowski, Mol. Biol. Cell, Jun 1999; 10: 1997. Melanie L. Styers, J. Cell Sci., Sep 2006; 119: 3643 - 3654. Qing-Fen Li, J. Biol. Chem., Nov 2006; 281: 34716 - 34724.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of total vimentin protein.
Subcellular Location	Cytoplasm

Uniprot	P08670
Gene Id	7431
Database Links	HGNC:12692 OMIM:116300 KEGG:hsa:7431 STRING:9606.ENSP00000224237 UniGene:Hs.455493
Function	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Protein Families	Intermediate filament family
Tissue Specificity	Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Weight	53,652 Da

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC

Description

ELISA ; WB ; IHC, WB ; ELISA ; IHC

Dilution

WB	1:500-1:3000
IHC	1:50-1:100

Tested Application

ELISA,WB,IHC;WB:1:500-1:3000,IHC:1:50-1:100

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10559789	CSB-PA233606	100ul	$297		Add to Cart

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