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Home / Primary Antibodies / IHC Antibodies

PIAS1 Antibody

SKU : BHA10559218

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA158229
Alternative Names	AR interacting protein; DDXBP1; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1; DEAD/H box-binding protein 1; GBP
Categories	Primary Antibodies
Clonality	polyclonal
Description	Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation By similarity.Bin Liu, Mol. Cell. Biol., Feb 2005; 25: 1113 - 1123. Hansol Lee, Genes & Dev., Apr 2006; 20: 784 - 794. Tamar Megidish, J. Biol. Chem., Mar 2002; 277: 8255 - 8259. Keiko Kawai-Kowase, Mol. Cell. Biol., Sep 2005; 25: 8009 - 8023.
Host	Rabbit
Immunogen	Synthesized peptide derived from Internal of Human PIAS1.
Raised In	Rabbit
Reactivity	Human, Mouse
Regulatory	RUO
Relevance	Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation By similarity. Bin Liu, Mol. Cell. Biol., Feb 2005; 25: 1113 - 1123. Hansol Lee, Genes & Dev., Apr 2006; 20: 784 - 794. Tamar Megidish, J. Biol. Chem., Mar 2002; 277: 8255 - 8259. Keiko Kawai-Kowase, Mol. Cell. Biol., Sep 2005; 25: 8009 - 8023.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of total PIAS1 protein.
Subcellular Location	Nucleus speckle, Nucleus, PML body

Uniprot	O75925
Database Links	HGNC:2752 OMIM:603566 KEGG:hsa:8554 STRING:9606.ENSP00000249636 UniGene:Hs.162458
Function	Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity).
Pathway	Jak-STAT signaling pathway Ubiquitin mediated proteolysis
Protein Families	PIAS family
Tissue Specificity	Expressed in numerous tissues with highest level in testis.

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC

Description

ELISA ; WB ; IHC, WB ; ELISA ; IHC

Dilution

WB	1:500-1:3000
IHC	1:50-1:100

Tested Application

ELISA,WB,IHC;WB:1:500-1:3000,IHC:1:50-1:100

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10559218	CSB-PA158229	100ul	$297		Add to Cart

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