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Home / Primary Antibodies / IHC Antibodies

Phospho-STAT1 (Ser727) Antibody

SKU : BHA10560550

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA225094
Alternative Names	ISGF-3; STAT91;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Yuan ZM, et al. (1999) Nature.399 (6738): 814-817.Schindler C, et al. (1992) Proc. Natl. Acad. Sci. U.S.A 89:7836-7839.Strausberg R.L (2002). Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903.Quelle F.W., (1995) J. Biol. Chem. 270:20775-20780.
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of serine 727 (P-M-S(p)-P-E) derived from Human STAT1.
Involvement In Disease	Immunodeficiency 31B (IMD31B); Immunodeficiency 31A (IMD31A); Immunodeficiency 31C (IMD31C)
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Yuan ZM, et al. (1999) Nature.399 (6738): 814-817. Schindler C, et al. (1992) Proc. Natl. Acad. Sci. U.S.A 89:7836-7839. Strausberg R.L (2002). Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903. Quelle F.W., (1995) J. Biol. Chem. 270:20775-20780.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of STAT1 only when phosphorylated at serine 727.
Subcellular Location	Cytoplasm, Nucleus

Uniprot	P42224
Database Links	HGNC:11362 OMIM:600555 KEGG:hsa:6772 STRING:9606.ENSP00000354394 UniGene:Hs.642990
Function	Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus
Pathway	Chemokine signaling pathway Jak-STAT signaling pathway Nitric oxide signaling MAPK Erk pathway Necroptosis NOD-like receptor signaling pathway Osteoclast differentiation Th1 and Th2 cell differentiation Th17 cell differentiation Toll-like receptor signaling pathway AGE-RAGE signaling pathway in diabetic complications
Protein Families	Transcription factor STAT family

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC, IF

Description

ELISA ; WB ; IHC ; IF, WB ; ELISA ; IHC ; IF

Dilution

WB	1:500-1:1000
IHC	1:50-1:100
IF	1:100-1:200

Tested Application

ELISA,WB,IHC,IF;WB:1:500-1:1000,IHC:1:50-1:100,IF:1:100-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560550	CSB-PA225094	100ul	$360		Add to Cart

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