Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Ferjoux G, et al. (2003) Mol Biol Cell. 2003 14(9): 3911-3928. Shi ZQ, et al. (2000) Mol Cell Biol 20(5): 1526-1536. Li C, Friedman JM. (1999) Proc Natl Acad Sci U S A 96(17): 9677-9682 Manes S, et al. (1999) Mol Cell Biol 19(4): 3125-3135. Oh ES, et al. (1999) Mol Cell Biol; 19(4): 3205-3215.
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of tyrosine 580 (R-V-Y(p)-E-N) derived from Human SHP-2.
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.
Ferjoux G, et al. (2003) Mol Biol Cell. 2003 ; 14(9): 3911-3928. Shi ZQ, et al. (2000) Mol Cell Biol ; 20(5): 1526-1536. Li C, Friedman JM. (1999) Proc Natl Acad Sci U S A ; 96(17): 9677-9682 Manes S, et al. (1999) Mol Cell Biol ; 19(4): 3125-3135. Oh ES, et al. (1999) Mol Cell Biol; 19(4): 3205-3215.
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous level of SHP-2 only when phosphorylated at tyrosine 580.
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway
Protein-tyrosine phosphatase family, Non-receptor class 2 subfamily
Tissue Specificity
Widely expressed, with highest levels in heart, brain, and skeletal muscle.
Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.