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Home / Primary Antibodies / IHC Antibodies

Phospho-PRKAA1/PRKAA2 (Ser487) Antibody

SKU : BHA10560623

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA261465
Alternative Names	AAPK1; AMPK alpha-1 chain; AMPK-alpha1; HMG-CoA redustase kinase; PRKAA1
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346. Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of serine 487 (S-G- SP-V-S) derived from Human AMPKa1.
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346. Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of AMPKa1 only when phosphorylated at serine 487.
Subcellular Location	Cytoplasm, Nucleus

Uniprot	Q13131
Database Links	HGNC:9376 OMIM:602739 KEGG:hsa:5562 STRING:9606.ENSP00000346148 UniGene:Hs.43322
Function	Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes
Pathway	mTOR signaling pathway PI3K-Akt signaling pathway Autophagy Adipocytokine signaling pathway AMPK signaling Apelin signaling pathway FoxO signaling pathway Glucagon signaling pathway Insulin signaling pathway
Protein Families	Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC, IF

Description

ELISA ; WB ; IHC ; IF, WB ; ELISA ; IHC ; IF

Dilution

WB	1:500-1:1000
IHC	1:50-1:100
IF	1:100-1:200

Tested Application

ELISA,WB,IHC,IF;WB:1:500-1:1000,IHC:1:50-1:100,IF:1:100-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560623	CSB-PA261465	100ul	$360		Add to Cart

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