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Home / Primary Antibodies / IHC Antibodies

Phospho-NFKBIA (Ser32/Ser36) Antibody

SKU : BHA10560547

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA093630
Alternative Names	I-kappa-B-alpha; IKBA; NF-kappaB inhibitor alpha; NFKBI; NFKBIA
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription. Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823.Honda, R. et al. (1997) FEBS Lett. 420, 25-27.Hirao, A. et al. (2000) Science 287, 1824-1827.Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157.Chehab,N.H.et al. (1999) Proc .Natl .Acad. Sci. USA 96, 13777-13782.
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IkB-a.
Involvement In Disease	Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant (ADEDAID)
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription. Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823. Honda, R. et al. (1997) FEBS Lett. 420, 25-27. Hirao, A. et al. (2000) Science 287, 1824-1827. Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157. Chehab,N.H.et al. (1999) Proc .Natl .Acad. Sci. USA 96, 13777-13782.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of IkB-a only when phosphorylated at serine 32/36.
Subcellular Location	Cytoplasm, Nucleus

Uniprot	P25963
Database Links	HGNC:7797 OMIM:164008 KEGG:hsa:4792 STRING:9606.ENSP00000216797 UniGene:Hs.81328
Function	Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Pathway	cAMP signaling pathway Chemokine signaling pathway NF-kappa B signaling pathway TNF signaling pathway Apoptosis B cell receptor signaling pathway IL-17 signaling pathway Inflammasome Signaling NOD-like receptor signaling pathway Osteoclast differentiation T cell receptor signaling pathway Th1 and Th2 cell differentiation Th17 cell differentiation Toll-like receptor signaling pathway Adipocytokine signaling pathway Neurotrophin signaling pathway
Protein Families	NF-kappa-B inhibitor family

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC, IF

Description

ELISA ; WB ; IHC ; IF, WB ; ELISA ; IHC ; IF

Dilution

WB	1:500-1:1000
IHC	1:50-1:100
IF	1:100-1:200

Tested Application

ELISA,WB,IHC,IF;WB:1:500-1:1000,IHC:1:50-1:100,IF:1:100-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560547	CSB-PA093630	100ul	$360		Add to Cart

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