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Home / Primary Antibodies / IHC Antibodies

Phospho-MARCKS (Ser170) Antibody

SKU : BHA10560584

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA116247
Alternative Names	MACS, 80K-L, PKCSL, PRKCSL
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. Ramsden, J.J. (2000) Int. J. Biochem. Cell Biol. 32, 475-479. Graff, J. M. et al. (1989) J. Biol. Chem. 264, 21818-21823. Hartwig, J. H. et al. (1992) Nature 356, 618-622. Thelen, M. et al. (1991) Nature 351, 320-322.
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of Serine 170 (G-F-S(p)-F-K) derived from Human MARCKS.
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. Ramsden, J.J. (2000) Int. J. Biochem. Cell Biol. 32, 475-479. Graff, J. M. et al. (1989) J. Biol. Chem. 264, 21818-21823. Hartwig, J. H. et al. (1992) Nature 356, 618-622. Thelen, M. et al. (1991) Nature 351, 320-322.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of MARCKS only when phosphorylated at threonine 170.
Subcellular Location	Cytoplasm, cytoskeleton, Membrane, Lipid-anchor

Uniprot	P29966
Database Links	HGNC:6759 OMIM:177061 KEGG:hsa:4082 STRING:9606.ENSP00000357624 UniGene:Hs.519909
Function	MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Pathway	Fc gamma R-mediated phagocytosis
Protein Families	MARCKS family

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC

Description

ELISA ; WB ; IHC, WB ; ELISA ; IHC

Dilution

WB	1:500-1:1000
IHC	1:50-1:100

Tested Application

ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:100

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560584	CSB-PA116247	100ul	$360		Add to Cart

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