Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Wang W, et al. (2002) Mol Cell Biol 22(10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55.
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of serine 189 (V-D-S(p)-V-A) derived from Human MKK3.
Involvement In Disease
Defects in MAP2K3 may be involved in colon cancer.
Raised In
Rabbit
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Relevance
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Wang W, et al. (2002) Mol Cell Biol ; 22(10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55.
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous level of MKK3 only when phosphorylated at serine189.
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
Pathway
MAPK signaling pathway TNF signaling pathway Cellular senescence Fc epsilon RI signaling pathway Toll-like receptor signaling pathway Rap1 signaling pathway
Protein Families
Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
Tissue Specificity
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.