Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Li BD, et al. (1998) Ann Surg; 227(5): 756-763 Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520 De Gregorio E, et al. (2001) RNA; 7(1): 106-113 Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E.
Involvement In Disease
Autism 19 (AUTS19)
Raised In
Rabbit
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Relevance
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Li BD, et al. (1998) Ann Surg; 227(5): 756-763 Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520 De Gregorio E, et al. (2001) RNA; 7(1): 106-113 Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous level of eIF4E only when phosphorylated at serine 209.
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.