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Home / Primary Antibodies / IHC Antibodies

PE Anti-Mouse CD34 Antibody[RAM34]

SKU : BHA10327392

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	E-AB-F1284UD
Background	The RAM34 antibody reacts with the CD34 glycoprotein on the surface of three independently derived mouse CD34-transfected cell lines. RAM34 antibody also reacts with the mouse cell lines PA6, 416B, Swiss 3T6, NIH, 3T3, DA1, and M1, all of which are positive for expression of mouse CD34 mRNA. Cell lines shown to be negative for CD34 transcript, including WEHI-3B, EL4, 18.8, and CMT64/61, are also negative for surface expression of CD34 as determined by RAM34 staining. Normal thymocytes and splenocytes are negative for CD34 expression. In the bone marrow, 7-10% of cells are stained with RAM34 mAb, including most of the Ly-6A/E (Sca-1)+ CD90 (Thy-1)low Lineage Marker- hematopoietic stem cell-enriched subpopulation and myeloerythroid progenitors. CD34 is also expressed on a small percentage of fetal liver cells, including NK-cell progenitors. CD34 has been reported to be expressed on the endothelium of capillaries and, in this form, to function as a ligand for L-selectin. Consistent with this observation, RAM34 antibody stains endothelial cells in spleen, thymus, and postcapillary HEVs in the lymph nodes. It is reported that RAM34 antibody can be used to select CD34+ CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker- bone marrow-derived hematopoietic stem cells, capable of short-term multi-lineage reconstitution of lethally irradiated mice; while the CD34- CD117+ Sca-1+ Lineage Marker- population contains self-renewing hematopoietic stem cells. Similarly, the bone marrow population with high dye-efflux capacity and which is highly enriched for long-term reconstituting hematopoietic stem cells is CD34- CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker-.
Categories	Primary Antibodies;Primary Antibodies///Flow Cytomerty(FACS)
Clonality	Monoclonal
Host	Rat
Isotype	Rat IgG2a, k
Reactivity	Mouse
Synonyms	Hematopoietic progenitor cell antigen CD34

Swissprot	Q64314
Accession Number	12490
Gene Id	12490
Clone No	RAM34

Buffer	PBS with 0.05% Proclin300, 1% BSA
Concentration	0.2 mg/mL
Storage	Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.

Applications	FCM

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SKU	Supplier No.	Size	Your price	Quantity
BHA10327392	E-AB-F1284UD	25ug	$79		Add to Cart
BHA10327392	E-AB-F1284UD	50ug	$125		Add to Cart
BHA10327392	E-AB-F1284UD	100ug	$200		Add to Cart

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