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Home / Primary Antibodies / IHC Antibodies

CHEK2 (Ab-68) Antibody

SKU : BHA10559120

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA884585
Alternative Names	CHEK2; CHK2; Cds1; Chk2; EC 2.7.11.1
Categories	Primary Antibodies
Clonality	polyclonal
Description	Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells.Bhoumik A, et al. (2005) Mol Cell; 18(5): 577-87.Gorgoulis VG, et al. (2005) Nature; 434(7035): 907-13.Falck J, et al. (2005) Nature; 434(7033): 605-11.Jin ZH, et al. (2005) Oncogene; 24(12): 1973-81.Li J, et al. (2005) J Biol Chem; 280(12): 12041-50.
Host	Rabbit
Immunogen	Synthesized non-phosphopeptide derived from Human Chk2 around the phosphorylation site of threonine 68.
Involvement In Disease	Li-Fraumeni syndrome 2 (LFS2); Prostate cancer (PC); Osteogenic sarcoma (OSRC); Breast cancer (BC)
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Bhoumik A, et al. (2005) Mol Cell; 18(5): 577-87. Gorgoulis VG, et al. (2005) Nature; 434(7035): 907-13. Falck J, et al. (2005) Nature; 434(7033): 605-11. Jin ZH, et al. (2005) Oncogene; 24(12): 1973-81. Li J, et al. (2005) J Biol Chem; 280(12): 12041-50.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of total Chk2 protein.
Subcellular Location	Isoform 2: Nucleus, Note=Isoform 10 is present throughout the cell, SUBCELLULAR LOCATION: Isoform 4: Nucleus, SUBCELLULAR LOCATION: Isoform 7: Nucleus, SUBCELLULAR LOCATION: Isoform 9: Nucleus, SUBCELLULAR LOCATION: Isoform 12: Nucleus, SUBCELLULAR LOCATION: Nucleus, PML body, Nucleus, nucleoplasm

Uniprot	O96017
Database Links	HGNC:16627 OMIM:114480 KEGG:hsa:11200 UniGene:Hs.291363
Function	Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition
Pathway	p53 signaling pathway Cell cycle Cellular senescence
Protein Families	Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CHK2 subfamily
Tissue Specificity	High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC

Description

ELISA ; WB ; IHC, WB ; ELISA ; IHC

Dilution

WB	1:500-1:3000
IHC	1:50-1:100

Tested Application

ELISA,WB,IHC;WB:1:500-1:3000,IHC:1:50-1:100

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10559120	CSB-PA884585	100ul	$297		Add to Cart

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