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Home / Primary Antibodies / IHC Antibodies

CDK4 Antibody

SKU : BHA10559082

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA275368
Alternative Names	PSK-J3; CMM3; CMM3
Categories	Primary Antibodies
Clonality	polyclonal
Description	Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle . Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681. Sherr, C.J. (1996) Science 274, 1672-1677. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925. Gabrielli, B.G. et al. (1999) J Biol Chem 274, 13961-9.
Host	Rabbit
Immunogen	Peptide sequence around aa.295~299(L-H-K-D-E)derived from Human CDK4.
Involvement In Disease	Melanoma, cutaneous malignant 3 (CMM3)
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle . Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681. Sherr, C.J. (1996) Science 274, 1672-1677. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925. Gabrielli, B.G. et al. (1999) J Biol Chem 274, 13961-9.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of total CDK4 protein.
Subcellular Location	Cytoplasm, Nucleus, Membrane

Uniprot	P11802
Database Links	HGNC:1773 OMIM:123829 KEGG:hsa:1019 STRING:9606.ENSP00000257904 UniGene:Hs.95577
Function	Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Pathway	p53 signaling pathway PI3K-Akt signaling pathway Cell cycle Cellular senescence T cell receptor signaling pathway AGE-RAGE signaling pathway in diabetic complications
Protein Families	Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC

Description

ELISA ; WB ; IHC, WB ; ELISA ; IHC

Dilution

WB	1:500-1:1000
IHC	1:50-1:200

Tested Application

ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10559082	CSB-PA275368	100ul	$297		Add to Cart

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