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Home / Primary Antibodies / IHC Antibodies

CCNB1 (Ab-147) Antibody

SKU : BHA10559670

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA973969
Categories	Primary Antibodies
Clonality	polyclonal
Description	The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470. Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685. Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.
Host	Rabbit
Immunogen	Peptide sequence around aa.145~149 (A-F-S-D-V) derived from Human Cyclin B1.
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470. Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685. Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of total Cyclin B1 protein.
Subcellular Location	Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Uniprot	P14635
Database Links	HGNC:1579 OMIM:123836 KEGG:hsa:891 STRING:9606.ENSP00000256442 UniGene:Hs.23960
Function	Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Pathway	p53 signaling pathway Cell cycle Cellular senescence FoxO signaling pathway
Protein Families	Cyclin family, Cyclin AB subfamily

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC, IF

Description

ELISA ; WB ; IHC ; IF, WB ; ELISA ; IHC ; IF

Dilution

WB	1:500-1:1000
IHC	1:50-1:200
IF	1:100-1:200

Tested Application

ELISA,WB,IHC,IF;WB:1:500-1:1000,IHC:1:50-1:200,IF:1:100-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10559670	CSB-PA973969	100ul	$297		Add to Cart

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