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Home / Primary Antibodies / IHC Antibodies

BUB1B Antibody

SKU : BHA10557272

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA002883KA01HU
Alternative Names	Bub1b; BUB1beta; BUBR1; Bub1A; MAD3L; SSK1; hBUBR1
Categories	Primary Antibodies
Description	The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions. During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/Cyclosome (APC/C), thereby preventing cells with unaligned chromosomes from prematurely entering anaphase. Research studies have shown that Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung, and breast cancers. Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome. Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes, and increased cancer susceptibility. Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores, and is required for functional microtubule-kinetochore interactions.
Hgnc	1149
Host	Rabbit
Immunogen	Recombinant protein of Human BUB1B
Intended Use	For research use only. Not for human, diagnostic or therapeutic use.
Involvement In Disease	Premature chromatid separation trait (PCS); Mosaic variegated aneuploidy syndrome 1 (MVA1)
Isotype	IgG
Mgi	1333889
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions. During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/Cyclosome (APC/C), thereby preventing cells with unaligned chromosomes from prematurely entering anaphase. Research studies have shown that Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung, and breast cancers. Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome. Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes, and increased cancer susceptibility. Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores, and is required for functional microtubule-kinetochore interactions.
Size	100μl(100μg)
Species	Homo Sapiens (Human)
Subcellular Location	Cytoplasm, Nucleus, Chromosome, centromere, kinetochore, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Rgd	619791
Target Details	This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Uniprot	O60566
Database Links	HGNC:1149 OMIM:176430 KEGG:hsa:701 STRING:9606.ENSP00000287598 UniGene:Hs.513645
Function	Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
Pathway	Cell cycle
Protein Families	Protein kinase superfamily, Ser/Thr protein kinase family, BUB1 subfamily
Tissue Specificity	Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.

Buffer	Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Format	liquid
Purification	Affinity purification
Purity	Affinity purification
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Storage Buffer	Store at -20oC or -80oC. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Applications

ELISA, WB, IHC

Description

ELISA ; WB ; IHC, WB ; ELISA ; IHC

Dilution

WB	1:500-1:2000
IHC	1:50-1:200

Tested Application

ELISA,WB,IHC;WB:1:500-1:2000,IHC:1:50-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10557272	CSB-PA002883KA01HU	100μl	$387.33		Add to Cart

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