Anti-undefined Antibody rACP5,1070 Unconjugated BHA10208303

Mfr.No.	54-MSM3-P1ABX
Alternative Names	Acid phosphatase 5, tartrate resistant ; ACP5 ; serum band 5 tartrate-resistant acid phosphatase ; SPENCDI ; Tartrate-resistant acid ATPase ; Tartrate-resistant acid phosphatase type 5 ; TR-AP ; TRACP5 ; TRAcP ; TRAP ; TrATPase ; Type 5 acid phosphatase
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	19p13.2
Clonality	monoclonal
Description	It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This MAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis. Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gaucher s Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.
Host	Mouse
Immunogen	Recombinant full-length human ACP5 protein
Isotype	IgG1
Positive Control	HepG2, 293T, K562 or RPMI-8226 Cells. Spleen from Hairy Cell Leukemia (HCL) patient.
Reactivity	Human, Mouse, Rat
Recombinant	TRUE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	P13686
Gene Id	54
Clone No	rACP5, 1070
Symbol	ACP5
Weight	35kDa

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., IHC-F

Dilution

Immunohistology (Formalin-fixed)

0.5-1μg/ml for 30 minutes at RT