Anti-undefined Antibody rVWF,1465 Unconjugated BHA10208312

Mfr.No.	7450-MSM8-P1ABX
Alternative Names	Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	12p13.31
Clonality	monoclonal
Description	von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
Host	Mouse
Immunogen	Recombinant fragment of human vWF protein (aa 1815-1939) (exact sequence is proprietary)
Isotype	IgG1
Positive Control	HUVEC cells or Tonsil
Reactivity	Human
Recombinant	TRUE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	P04275
Gene Id	7450
Clone No	rVWF, 1465
Symbol	VWF
Weight	250kDa

Buffer	200μg/ml of recombinant Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

IF, WBCLIP, IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., IF ; WBCLIP ; IHC-F

Dilution

Immunofluorescence	0.5-1μg/ml
Western Blotting	0.5-1.0μg/ml
Immunoprecipitation	0.5-1µg/500ug protein lysate
Immunohistology (Formalin-fixed)	1-2μg/ml for 30 minutes at RT