Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
Host
Mouse
Immunogen
Recombinant fragment of human BL-CAM/CD22 protein (around aa 52-178) (exact sequence is proprietary)
Isotype
IgG1 Kappa
Positive Control
Raji, Daudi, IM9, JY25 and human peripheral blood lymphocytes. Human Tonsil, Lymph Node or Spleen.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
P20273
Gene Id
933
Clone No
BLCAM, 1796
Symbol
CD22
Weight
130-140kDa
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
ELISA, IHC-F
Description
Order Ab without BSA, Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., ELISA ; IHC-F
Dilution
ELISA
Use Ab at 2-4μg/ml for coating
Immunohistology (Formalin-fixed)
1-2μg/ml for 30 minutes at RT
No Description Available.
No Description Available.
No Description Available.
No Description Available.
Reviews of Anti-undefined Antibody BLCAM,1796 Unconjugated BHA10208110