Anti-undefined Antibody rBCL6,1527 Unconjugated BHA10208080

Mfr.No.	604-MSM10-P1ABX
Alternative Names	B-cell lymphoma 5 protein ; B-Cell Lymphoma 6 Protein ; BCL5 ; BCL6 ; BCL6A ; cys his2 zinc finger transcription factor ; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3) ; Zinc finger and BTB domain-containing protein 27 (ZBTB27) ; Zinc Finger Protein 51 (ZNF51) ; zinc finger transcription factor BCL6S
Categories	Primary Antibodies
Cellular Localization	Nuclear
Chromosome Location	3q27.3
Clonality	monoclonal
Description	The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ( L&H ) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.
Host	Mouse
Immunogen	Recombinant fragment of human bcl-6 protein (around aa 256-389) (exact sequence is proprietary)
Isotype	IgG1 Kappa
Positive Control	Raji or Ramos cells. Tonsil or Hodgkin s lymphoma.
Reactivity	Human
Recombinant	TRUE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	P41182
Gene Id	604
Clone No	rBCL6, 1527
Symbol	BCL6
Weight	95kDa

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., IHC-F

Dilution

Immunohistology (Formalin-fixed)

1-2μg/ml for 30 minutes at RT