Anti-CD22 / BL-CAM Antibody BHA10206391

Mfr.No.	933-MSM5-P1ABX
Alternative Names	B-lymphocyte cell adhesion molecule (BL-CAM) ; B-cell receptor CD22 ; CD22 ; Lectin 2 ; Lyb8 ; Sialic acid-binding Ig-like lectin 2 (Siglec-2) ; SIGLEC2 ; T-cell surface antigen Leu-14
Categories	Primary Antibodies
Cellular Localization	Cell Surface
Chromosome Location	19q13.1
Clonality	Monoclonal
Description	Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
Host	Mouse
Immunogen	Recombinant human BL-CAM/CD22 protein (aa52-178) (exact sequence is proprietary)
Positive Control	Raji, Daudi, IM9, JY25 and human peripheral blood lymphocytes. Human Tonsil
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P20273
Uniprot	579691
Gene Id	933

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

ELISA, FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., ELISA ; FACS ; IF ; WB ; IHC-F

Dilution

ELISA	Use Ab at 2-4μg/ml for coating
FACS	0.5-1μg/million cells
IF	0.5-1μg/ml
WB	0.5-1.0μg/ml
IHC-F	1-2μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10206391	933-MSM5-P1ABX	100µg	$393.20		Add to Cart

Anti-CD22 / BL-CAM Antibody BHA10206391

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