Anti-Nuclear Mitotic Apparatus Protein (NuMA) Antibody BHA10203874

Mfr.No.	4926-MSM1X-P1ABX
Alternative Names	Nuclear Mitotic Apparatus Protein 1 ; NuMA protein ; NUMA1 ; SP-H antigen ; Structural nuclear protein
Categories	Primary Antibodies
Cellular Localization	Nuclear
Chromosome Location	11q13
Clonality	Monoclonal
Description	Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.
Host	Mouse
Immunogen	Colon carcinoma 174T cells
Isotype	IgM Kappa
Positive Control	Exponentially growing any cultured human cells. Tonsil or lymph node.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	Q14980
Uniprot	325978
Gene Id	4926

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml
IHC-F	0.5-1μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10203874	4926-MSM1X-P1ABX	100µg	$393.20		Add to Cart

Anti-Nuclear Mitotic Apparatus Protein (NuMA) Antibody BHA10203874

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