Anti-CD31 / PECAM-1 Antibody BHA10204030

Mfr.No.	5175-MSM4-P1ABX
Alternative Names	EndoCAM ; PECA1 ; Platelet Endothelial Cell Adhesion Molecule 1 ; GPIIA'
Categories	Primary Antibodies
Cellular Localization	Cell Surface, Cytoplasm of endothelial cells
Chromosome Location	17q23.3
Clonality	Monoclonal
Description	CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Host	Mouse
Immunogen	Membrane preparation of a spleen from a patient with hairy cell leukemia
Isotype	IgG1 Kappa
Positive Control	Jurkat cells. Tonsil or Angiosarcoma.
Reactivity	Human, Cynomolgus Monkey, Rabbit
Recombinant	FALSE
Regulatory	RUO

Swissprot	P16284
Uniprot	376675; 514412
Gene Id	5175

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution