Anti-Neurofilament (NF-H) Antibody BHA10203776

Mfr.No.	4744-MSM2-P0
Alternative Names	NEFH ; Neurofilament H ; Neurofilament Heavy Polypeptide 200kDa ; Neurofilament Triplet H Protein ; NF-H ; NF200
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	22q12.2
Clonality	Monoclonal
Description	This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Host	Mouse
Immunogen	Triton-X 100 insoluble protein fraction of rat brain
Isotype	IgG1
Positive Control	Brain, Neuroblastoma
Reactivity	Human, Mouse, Rat, Chicken, Pig
Recombinant	FALSE
Regulatory	RUO

Swissprot	P12036
Uniprot	198760
Gene Id	4744

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200µg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml

Applications

FACS, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IHC-F

Dilution