Anti-Myogenin Antibody BHA10203682

Mfr.No.	4656-MSM2X-P1ABX
Alternative Names	bHLHc3 ; cb553 ; Class C basic helix-loop-helix protein 3 ; Myf-4 ; MYF4 ; MYOG ; Myogenic factor 4 ; Myogenin myogenin factor 4
Categories	Primary Antibodies
Cellular Localization	Nuclear
Chromosome Location	1q32.1
Clonality	Monoclonal
Description	Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms� tumor.
Host	Mouse
Immunogen	Human myogenin recombinant protein
Isotype	IgG1 Kappa
Positive Control	Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.
Reactivity	Human, Mouse, Rat, Cat, Pig
Recombinant	FALSE
Regulatory	RUO

Swissprot	P15173
Uniprot	2830
Gene Id	4656

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

ELISA, FACS, IF, WB, IP, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., ELISA ; FACS ; IF ; WB ; IP ; IHC-F

Dilution

ELISA	For coating, order Ab without BSA
FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml
WB	0.5-1.0μg/ml
IP	0.5-1 �g/500ug protein Lysate
IHC-F	0.5-1.0μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10203682	4656-MSM2X-P1ABX	100µg	$393.20		Add to Cart

Anti-Myogenin Antibody BHA10203682

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