Apomucin Major Airway Glycoprotein ; Mucin 5 subtype AC tracheobronchial ; Mucin 5 Subtypes A And C ; Mucin 5AC oligomeric mucus/gel forming ; Tracheobronchial Mucin (TBM)
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic
Chromosome Location
11p15.5
Clonality
Monoclonal
Description
This MAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
Host
Mouse
Immunogen
M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
Isotype
IgG1 Kappa
Positive Control
Stomach
Reactivity
Human, Monkey, Rabbit, Cat, Mouse, Rat, Pig, Hedgehog, Chicken
Recombinant
FALSE
Regulatory
RUO
Swissprot
P98088
Uniprot
534332
Gene Id
4586
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
1-2μg/ml
IHC-F
1-2μg/ml for 30 minutes at RT
Reviews of Anti-MUC5AC (Mucin 5AC / Gastric Mucin) Antibody BHA10203505