Anti-CD50 / ICAM-3 Antibody BHA10202033

Mfr.No.	3385-MSM3-P1ABX
Alternative Names	ICAMR ; Intercellular adhesion molecule 3 (ICAM3)
Categories	Primary Antibodies
Cellular Localization	Cell Surface
Chromosome Location	19p13.2
Clonality	Monoclonal
Description	Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues.
Host	Mouse
Immunogen	Stimulated human leukocytes
Isotype	IgG2a Kappa
Positive Control	HL-60 or THP-1 cells. Lymph node and tonsil
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P32942
Uniprot	354563
Gene Id	3385

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin/paraffin tissues Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml
IHC-F	0.5-1μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10202033	3385-MSM3-P1ABX	100µg	$393.20		Add to Cart

Anti-CD50 / ICAM-3 Antibody BHA10202033

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