Anti-EMI1 (Early Mitotic Inhibitor-1) Antibody BHA10201424

Mfr.No.	26271-MSM1-P1ABX
Alternative Names	Early Mitotic Inhibitor-1 (EMI1) ; FBX5 ; Fbxo31
Categories	Primary Antibodies
Cellular Localization	Nuclear
Chromosome Location	6q25.2
Clonality	Monoclonal
Description	It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Host	Mouse
Immunogen	Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
Isotype	IgG2a Kappa
Positive Control	HeLa, 293 or HepG2 cells. Ovarian carcinoma.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	Q9UKT4
Uniprot	520506
Gene Id	26271

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution