Anti-Clathrin, Light Chain Antibody BHA10200502

Mfr.No.	1211-MSM1-P1ABX
Alternative Names	Clathrin light chain A ; Clathrin light chain B ; Clathrin light chain LCA ; Clathrin light chain LCB ; Clathrin light polypeptide A ; Clathrin light polypeptide B ; Clathrin, light polypeptide (Lca) ; Clathrin, light polypeptide (Lcb) ; CLTA ; CLTB
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	9p13.3
Clonality	Monoclonal
Description	Recognizes proteins of 31-44kDa, which are identified as Clathrin Light Chains (both A & B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60% homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.
Host	Mouse
Immunogen	Purified recombinant N-terminal fragment of human Clathrin Light Chain
Isotype	IgG2b Kappa
Positive Control	HeLa cells. Placenta or Prostate Carcinoma.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P09496 and P09497
Uniprot	484241 and 522114
Gene Id	1211;1212

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

FACS	0.5-1μg/million cells
IF	1-2μg/ml
WB	0.5-1μg/ml
IHC-F	0.5-1μg/ml for 30 min at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10200502	1211-MSM1-P1ABX	100µg	$393.20		Add to Cart

Anti-Clathrin, Light Chain Antibody BHA10200502

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