Human Recombinant GRP78 (Bip) Protein

Mfr.No.	SPR-107, SPR-107A, SPR-107B
Description	Active Human Recombinant GRP78 (Bip) Protein
Alternative Names	BIP Protein, Grp78 Protein, HSPA5 Protein, MIF2 Protein, immunoglobulin heavy chain binding Protein
Biological Activity	ATPase active
Cellular Localization	Endoplasmic Reticulum Lumen, Melanosome
Certificate Of Analysis	This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 2.3uM phosphate liberated/hr/ug protein in a 200ul reaction at 37°C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
Regulatory	RUO
Scientific Background	GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia (Barnes), to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
Species	Human
Specificity	~78 kDa
Accession Number	NM_005347
Concentration	Lot/batch specific. See included datasheet.
Purification	Affinity Purified
Expression System	E. coli
Amino Acid Sequence	MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL
Bioactivity	ATPase active
Biological Activity Abreviation	Active
Biological Activity Comment	ATPase active
Cite This Product	Human Recombinant GRP78 (Bip) Protein (StressMarq Biosciences, Canada, Cat # SPR-107A)
Mol Weight	~78 kDa
Nature	Recombinant

Gross Weight (kg)	1.4
Launch Date	2007/09/01
Net Weight (g)	0.05
Number Of Pubmed Citations	4
Swissprot	P11021
Target Details	GRP78 (Bip)
Gene Id	3309
Research Area	Cancer, Heat Shock, Cell Signaling, Organelle Markers, Chaperone Proteins, Protein Trafficking
Antigen	GRP78 (Bip)
Target	GRP78 (Bip)

Adr Code	Non-hazardous
Country Of Origin	Canada
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Published Application	Western Blot ; ELISA ; ATPase activity assay ; Western Blot Control
Published Species Reactivity	Human ; Mouse ;
Pubmed Id	28026090 ; 24464222 ; 24512104 ; 19209902
Shipping	Blue Ice or 4ºC
Stock Product	Y
Tarriff Code	3822.19.0030
Un Code For Transport	Non-hazardous
Unspsc Code	12352202
Shipping Temperature	Blue Ice or 4°C
Buffer	50mM Tris, 150mM NaCl, 10% glycerol
Description	Accession Number: NM_005347 Specificity: ~78 kDa Certificate of Analysis: This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 2.3µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay. Country of Origin: Canada Gross Weight (kg): 1.4 Net Weight (g): 0.05 Stock Product: Y
Purity	>90%
Storage Buffer	50mM Tris/HCl, pH 7.5, 150mM NaCl, 10% glycerol
Storage Temperature	-20°C

Immunogen Species	Homo Sapiens (Human)
Applications	ATPase Activity assay, Functional assay, WB, ELISA, SDS-PAGE

Reference	1. Cho S., et al (2007) Mol Cell Biol. 27(1): 368-83. 2. Yang Y., et al. (1998) J Biol Chem. 273: 25552-25555. 3. Luo S., et al (2006) 26(15): 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19: 4333-4336. 6. Laquerre S., et al. (1998) J. Virology. 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res. 65(13): 5785-91.

Product Manual	Datasheet
SDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP11900019-1	SPR-107A	50 ug	$159		Add to Cart
BHP11900019-2	SPR-107B	100 ug	$299		Add to Cart

Human Recombinant GRP78 (Bip) Protein

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