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Home / Primary Antibodies / Monoclonal Antibodies

HSP90 alpha/beta Antibody, Clone Hyb-K41220A (SMC-135)

BHA11900480
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Antigen HSP90 alpha, HSP90 beta
Swissprot P08238, P07900
Accession Number NP_031381.2, NP_001017963.2
Gene Id 3326, 3320
Target HSP90 alpha/beta
Clone No Hyb-K41220A
Research Area Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Tumor Biomarkers, Protein Trafficking
Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms alpha and beta, which share 85% sequence amino acid homology. The two isoforms of HSP90 are expressed in the cytosolic compartment (1). Despite the similarities, HSP90alpha exists predominantly as a homodimer while HSP90beta exists mainly as a monomer (2). From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (3-6). Furthermore, HSP90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively.
HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation.
In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (9).
Shipping Temperature Blue Ice or 4°C
Specificity Detects 90kDa. Will detect both alpha (inducible) and beta (constitutively-expressed) forms.
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications ELISA, IHC, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; ELISA
Dilution WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Nemoto, T. et al. (1997) J.Biol Chem. 272: 26179-26187.

2. Minami Y, et al. (1991), J.Biol Chem. 266: 10099-10103.

3. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577.

4. Pearl H, et al. (2001) Adv Protein Chem 59: 157-186.

5. Neckers L, et al. (2002) Trends Mol Med 8: S55-S61.

6. Pratt W, Toft D. (2003) Exp Biol Med 228: 111-133.

7. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360.

8. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434.

9. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328.

10. Kishimoto J, et al. (2005). Cell Stress and Chaperones. 10 (4): 296-311.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900480-1 SMC-135D Unconjugated 100 ug $352
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BHA11900480-2 SMC-135D-A390 ATTO 390 100 ug $407
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BHA11900480-3 SMC-135D-A488 ATTO 488 100 ug $406
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BHA11900480-4 SMC-135D-A594 ATTO 594 100 ug $406
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BHA11900480-5 SMC-135D-BI Biotin 100 ug $402
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BHA11900480-6 SMC-135D-FITC FITC 100 ug $397
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BHA11900480-7 SMC-135S Unconjugated 12 ug $45
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