Matrices listed below were spiked with certain level of TNF-alpha and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-alpha in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
80-102
90
EDTA plasma(n=5)
86-103
92
heparin plasma(n=5)
88-100
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
81-103%
81-97%
90-105%
EDTA plasma(n=5)
90-101%
90-101%
94-98%
heparin plasma(n=5)
91-99%
92-98%
91-100%
Sensitivity
< 0.938pg/ml
Notes
For Research Use Only
UniProt ID
P06804
Applications
ELISA
Applications Notes
TNF-alpha ELISA Kit allows for the in vitro quantitative determination of TNF-alpha concentrations in serum, plasma, tissue homogenates and other biological fluids.