Matrices listed below were spiked with certain level of NSE and the recovery rates were calculated by comparing the measured value to the expected amount of NSE in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-104
97
EDTA plasma(n=5)
88-99
94
heparin plasma(n=5)
85-100
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NSE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-104%
86-99%
90-99%
EDTA plasma(n=5)
89-100%
90-97%
86-101%
heparin plasma(n=5)
82-95%
82-94%
80-100%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
P09104
Applications
ELISA
Applications Notes
NSEELISA Kit allows for the in vitro quantitative determination of NSE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human NSE(Neuron-specific Enolase)ELISA Kit