Matrices listed below were spiked with certain level of IL-33 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-33 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
95
EDTA plasma(n=5)
89-99
94
heparin plasma(n=5)
85-101
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-33 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-104%
86-104%
86-103%
EDTA plasma(n=5)
85-99%
90-97%
85-101%
heparin plasma(n=5)
85-100%
86-98%
87-92%
Sensitivity
< 1.875pg/ml
Notes
For Research Use Only
UniProt ID
O95760
Applications
ELISA
Applications Notes
IL-33 ELISA Kit allows for the in vitro quantitative determination of IL-33 concentrations in serum, plasma, tissue homogenates and other biological fluids.