Mouse Anti-Human GFAP R416WT Monoclonal IgG1

Mfr.No.	SMC-442, SMC-442D, SMC-442D-A390, SMC-442D-A488, SMC-442D-A594, SMC-442D-APC, SMC-442D-BI, SMC-442D-FITC, SMC-442D-HRP, SMC-442D-PCP, SMC-442D-RPE, SMC-442S
Immunogen Species	Human
Alternative Names	Glial fibrillary acidic protein Antibody, Intermediate filament protein Antibody, Astrocyte Antibody, gfapl Antibody, DKFZp459C0729 Antibody, MGC139638 Antibody, FLJ45472 Antibody, AI836096 Antibody, GFAP Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm
Certificate Of Analysis	1 ug/ml of SMC-442 was sufficient for detection of GFAP R416WT in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Description	Mouse Anti-Human GFAP R416WT Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP; 100% identical in rat and mouse. >50% identity with other proteins (Vimentin, Desmin and Peripherin)., Homo Sapiens (Human)
Immunogen Region	Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP; 100% identical in rat and mouse. >50% identity with other proteins (Vimentin, Desmin and Peripherin).
Isotype	IgG1
Launch Date	2013/10/28
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Human

Antigen	GFAP
Swissprot	P14136
Accession Number	NP_001124491.1
Gene Id	2670
Target	GFAP
Clone No	S206B-9
Research Area	Cell Markers, Glial Markers, Astrocyte Markers, Neuroscience

Buffer	PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~50kDa. Does not cross-react with GFAP-R416W or other proteins (based on KO validation results).
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IHC, WB, ICC/IF
Description	WB ; IHC ; ICC ; IF
Dilution	WB (1:1000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Velasco M.E., et al. (1980) Cancer. 45:484. 2. Bonnin J.M., et al. (1984) Acta Neuropathology. 62:185. 3. Lee VM-Y., et al. (1984) J. Neurochem. 42:25-32 (1984). 4. Trojanowski JQ et al. (1986) J. Neurochem. 6(3): 650-660 (1986). 5. Schmidt ML et al; Lab Invest 56:282-294 (1987). 6. Kosik KS et al; Neuron 1:817-825 (1988). 7. Schmidt ML et al; Lab Invest 59:460-466 (1988). 8. Mokuna, K, et al: J Neurosci Res 23:396 (1989). 9. Molenaar, et al; Exp Neurology 108:1-9 (1990). 10. Tohyama T et al; Am J Pathol, 142:871-882 (1993). 11. Tohyama T et al; Am J Pathol 142:883-892 (1993). 12. Thilenius, A.R.B., et al; J. Immunol. 162(2): 643-650 (1999).

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11904305-1	SMC-442D	Unconjugated	100 ug	$337	Add to Cart
BHA11904305-2	SMC-442D-A390	ATTO 390	100 ug	$392	Add to Cart
BHA11904305-3	SMC-442D-A488	ATTO 488	100 ug	$391	Add to Cart
BHA11904305-4	SMC-442D-A594	ATTO 594	100 ug	$391	Add to Cart
BHA11904305-5	SMC-442D-APC	APC	100 ug	$390	Add to Cart
BHA11904305-6	SMC-442D-BI	Biotin	100 ug	$386	Add to Cart
BHA11904305-7	SMC-442D-FITC	FITC	100 ug	$381	Add to Cart
BHA11904305-8	SMC-442D-HRP	HRP	100 ug	$377	Add to Cart
BHA11904305-9	SMC-442D-PCP	PerCP	100 ug	$390	Add to Cart
BHA11904305-10	SMC-442D-RPE	RPE	100 ug	$388	Add to Cart
BHA11904305-11	SMC-442S	Unconjugated	12 ug	$45	Add to Cart

GFAP R416WT Antibody, Clone N206B/9 (SMC-442)