General species Hyaluronic Acid (HA) (Competitive EIA) ELISA Kit

Mfr.No.

MEA182Ge

Sensitivity

1.77ng/mL

Detection Range

4.94-400ng/mL

Reactivity

General

Assay Duration

Assay Type

Competitive Inhibition

Sample Volume

15-25 ul(Please refer to the manual for the specific data.)

Description

This assay has high sensitivity and excellent specificity for detection of Mini Samples Hyaluronic Acid (HA).
No significant cross-reactivity or interference between Mini Samples Hyaluronic Acid (HA) and analogues was observed.

Alternative Names

Hyaluronan; Hyaluronate

Detection Method

Colormetric

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×60µL	Assay Diluent A	1×6mL
Detection Reagent B	1×60µL	Assay Diluent B	1×6mL
TMB Substrate	1×9mL	Stop Solution	1×3mL
Wash Buffer (30 × concentrate)	1×10mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of Mini Samples Hyaluronic Acid (HA) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Hyaluronic Acid (HA) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	90-103	97
EDTA plasma(n=5)	78-89	84
heparin plasma(n=5)	80-97	80

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Hyaluronic Acid (HA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	96-104%	80-92%	83-92%	79-98%
EDTA plasma(n=5)	98-105%	90-101%	97-105%	80-94%
heparin plasma(n=5)	82-93%	78-94%	90-104%	80-93%

Features

Detects protein (micro-volume samples 15-25 ?l)

Species

General species

Sample Type

Serum, Plasma, Other biological fluids

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Hyaluronic Acid (HA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Hyaluronic Acid (HA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Hyaluronic Acid (HA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Hyaluronic Acid (HA) and unlabeled Mini Samples Hyaluronic Acid (HA) (Standards or samples) with the pre-coated antibody specific to Mini Samples Hyaluronic Acid (HA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Hyaluronic Acid (HA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Hyaluronic Acid (HA) in the sample.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
And then add 25?L prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.

Tissue Specificity	Reactive with General species HA / Hyaluronic Acid

Applications	ELISA
Research Area	Tumor immunity

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10109766	MEA182Ge	96T*1	$955	Add to Cart
BHE10109766	MEA182Ge	96T*5	$4297.50	Add to Cart
BHE10109766	MEA182Ge	96T*10	$8117.50	Add to Cart

Mini Samples ELISA Kit for Hyaluronic Acid (HA) (General species)

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