EasyStep Human IL6(Interleukin 6) ELISA Kit

Mfr.No.

ELK001ES

Sensitivity

0.1pg/ml

Detection Range

1.56-100 pg/mL

Reactivity

Human

Assay Duration

1.5h

Assay Type

Sandwich

Alternative Names

IL-6;MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor

Recovery

Matrices listed below were spiked with certain level of recombinant IL6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL6 in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	95-110%	100%
Heparin plasma(n=5)	93-102%	98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-98%	98-110%	99-116%	95-110%
EDTA plasma(n=5)	87-102%	85-95%	85-98%	82-95%
Heparin plasma(n=5)	88-102%	95-106%	95-108%	96-115%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
100.00	2.999	2.939
50.00	2.373	2.313
25.00	1.683	1.623
12.50	1.117	1.057
6.25	0.708	0.648
3.13	0.344	0.284
1.57	0.175	0.115
0.00	0.060	0.000

Sample Type

Saliva, Cell lysates, Serum, Cell culture supernatants, Tissue homogenates, Urine, Cerebrospinal fluid, Plasma, Other biological fluids

Limit Of Detection

100 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P05231

Applications	ELISA
Research Area	Cytokine;Tumor immunity;Infection immunity;Cardiovascular biology;

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15208324	ELK001ES	48T	$320		Add to Cart
BHE15208324	ELK001ES	96T	$458		Add to Cart

EasyStep Human IL6(Interleukin 6) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of EasyStep Human IL6(Interleukin 6) ELISA Kit

Rate it now..!!