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Fine Test
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Fine Test
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| Mfr.No. | EH1465 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | 0.188ng/ml | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Detection Range | 0.313-20ng/ml | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Reactivity | Human | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Assay Duration | 4 hours | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Assay Type | Sandwich | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Description | This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti FOS antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with FOS bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of FOS in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Alternative Names | Protein c-Fos ELISA Kit, Cellular oncogene fos ELISA Kit, Fos proto-oncogene, AP-1 transcription factor subunit ELISA Kit, G0/G1 switch regulatory protein 7 ELISA Kit, Proto-oncogene c-Fos ELISA Kit, Transcription factor AP-1 subunit c-Fos ELISA Kit, FOS ELISA Kit, G0S7 ELISA Kit | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Detection Method | Double Antibody | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Kit Components |
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| Recovery |
Add a certain amount of FOS into the sample. Calculate the recovery by comparing the measured value with the expected amount of FOS in the sample.
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| Species | Human | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Specificity | Specifically binds with FOS , no obvious cross reaction with other analogues. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Standard Curve |
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.) Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
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| Sample Type | Tissue lysate, Cell lysates, Other biological fluids | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Assay Procedure Summary |
 Step 1:Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37°C. Washing:Wash the plate twice without immersing. Step 2:Add 100ul biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37°C. Washing:Wash the plate three times and immerse for 1min each time. Step 3:Add 100ul HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37°C. Washing:Wash the plate five times and immerse for 1min each time. Step 4:Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.) Step 5:Add 50ul stop solution. Read at 450nm immediately and calculate. |
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| Components | Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Precision |
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate. Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
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| Background | FOS/Proto-oncogene c-Fos/Cellular oncogene fos/G0/G1 switch regulatory protein 7/G0S7 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Stability |
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
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| Notes |
For Research Use Only Required Instruments And Reagents
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| Usage Notes |
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