General speciesCLIA Kit for Polyethylene Glycol (PEG) CLIA (Competitive)

Mfr.No.

CCX163Ge

Sensitivity

1.5ng/mL

Detection Range

3.9-1,000ng/mL

Reactivity

General

Assay Duration

Assay Type

Competitive Inhibition

Sample Volume

50 ul(Please refer to the manual for the specific data.)

Description

This assay has high sensitivity and excellent specificity for detection of Polyethylene Glycol (PEG).
No significant cross-reactivity or interference between Polyethylene Glycol (PEG) and analogues was observed.

Alternative Names

PEO; POE; Polyethylene oxide; Polyoxyethylene; Carbowax; GoLYTELY; GlycoLax; Fortrans; TriLyte; Colyte; Halflytely; Macrogol; MiraLAX; MoviPrep

Detection Method

Chemiluminescence

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Substrate A	1×10mL	Substrate B	1×2mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Polyethylene Glycol (PEG) and the recovery rates were calculated by comparing the measured value to the expected amount of Polyethylene Glycol (PEG) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	93-103	99
EDTA plasma(n=5)	93-102	98
heparin plasma(n=5)	86-95	92

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Polyethylene Glycol (PEG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	90-98%	97-105%	82-90%	78-89%
EDTA plasma(n=5)	94-105%	87-95%	85-93%	80-88%
heparin plasma(n=5)	78-91%	83-91%	90-99%	85-101%

Features

Detects small molecule

Species

General species

Sample Type

Cell lysates, Cell culture supernates, Serum, Tissue homogenates, Plasma, Other biological fluids

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Polyethylene Glycol (PEG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Polyethylene Glycol (PEG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Polyethylene Glycol (PEG). A competitive inhibition reaction is launched between biotin labeled Polyethylene Glycol (PEG) and unlabeled Polyethylene Glycol (PEG) (Standards or samples) with the pre-coated antibody specific to Polyethylene Glycol (PEG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Polyethylene Glycol (PEG) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Polyethylene Glycol (PEG) level in the sample or standard.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

Tissue Specificity	Reactive with General species PEG / Polyethylene Glycol

Applications	ELISA

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10110022	CCX163Ge	96T*1	$1080	Add to Cart
BHE10110022	CCX163Ge	96T*5	$4860	Add to Cart
BHE10110022	CCX163Ge	96T*10	$9180	Add to Cart

CLIA Kit for Polyethylene Glycol (PEG) (General species)

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