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Home / Fish Probe

eFISH PML/RARA Dual Color Dual FUSI

SKU : BHR15100181

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BioGenex Laboratories

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Mfr.No.	FP073-20X
Gene Name	PML/RARA
Fluorophore	Orange/Green
Chromosome	17
Intended Use	The BioGenex eFISH PML/RARA Dual color dual fusion probe is currently available forResearch use only.eFISH PML/RARA Dual color dual fusion probe is designed to beusedfor the detection of the translocationt(15;17)(q24;q21) in formalin-fixed,paraffinembeddedtissue or cells by fluorescence in situhybridization (FISH).BioGenex eFISH PML/RARA Dual color dual fusion probe comes in hybridizationbuffercontains orange-labeled polynucleotides(Orange: excitation at 547 nm and emissionat572 nm, similar to rhodamine), which target thePML gene in 15q24.1, and green-labeledpolynucleotides(Green: excitation at 503 nm andemission at 528 nm, similar to FITC),whichtarget the RARA gene in 17q21.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for thedetection of chromosomal aberrations, presence or absence of specific DNA sequence innative context. In this technique florescent probes bind to the target sequence of DNA inchromosome. High specificity and sensitivity coupled rapid and an accurate result hasproven role of FISH in both research and diagnosis of solid tumor and hematologicalmalignancies. As technique of cancer cytogenetics, FISH, can be used to identify geneticaberrations viz., deletions, amplification and translocation in tissue sections or within individual cells. FISH is also used for use in genetic counseling, medicine, and speciesidentification. FISH can also be used to detect and localize specific RNA targets in cells,circulating tumor cells, and tissue samples1,2,3,4,5.In an FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/antifade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acidsequences directly within a cell or tissue. High specificity is ensured through the action ofannealing of fluorescence probe nucleic acid sequence to complementary target nucleicacid sequence. ISH techniques can be used to identify genetic aberrations like deletions,amplification, translocationin tissue sections or within individual cells.
Storage	The BioGenex eFISH PML/RARA dual color dual fusion probe must be stored at 2-8°C protected from light and is stable through the expiry date printed on the label.

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100181	FP073-20X	20 Test	$1793		Add to Cart

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